• Abscisic acid regulates anthocyanin biosynthesis and gene expression associated with cell wall modification in ripening bilberry (vaccinium myrtillus l.) fruits 

      Karppinen, Katja Hannele; Tegelberg, Pinja; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-08-29)
      Ripening of non-climacteric bilberry (Vaccinium myrtillus L.) fruit is characterized by a high accumulation of health-beneficial anthocyanins. Plant hormone abscisic acid (ABA) and sucrose have been shown to be among the central signaling molecules coordinating non-climacteric fruit ripening and anthocyanin accumulation in some fruits such as strawberry. Our earlier studies have demonstrated an ...
    • Analysis of composition, morphology, and biosynthesis of cuticular wax in wild type bilberry (Vaccinium myrtillus L.) and its glossy mutant 

      Trivedi, Priyanka; Nguyen, Nga; Klavins, Linards; Kviesis, Jorens; Heinonen, Esa; Remes, Janne; Jokipii-Lukkari, Soile; Klavins, Maris; Karppinen, Katja; Jaakola, Laura; Häggman, Hely (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-09)
      In this study, cuticular wax load, its chemical composition, and biosynthesis, was studied during development of wild type (WT) bilberry fruit and its natural glossy type (GT) mutant. GT fruit cuticular wax load was comparable with WT fruits. In both, the proportion of triterpenoids decreased during fruit development concomitant with increasing proportions of total aliphatic compounds. In GT fruit, ...
    • Anthocyanin Profile in Berries of Wild and Cultivated Vaccinium spp. along Altitudinal Gradients in the Alps 

      Zoratti, Laura; Jaakola, Laura; Häggman, Hely; Giongo, Lara (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-09-16)
      Vaccinium spp. berries provide some of the best natural sources of anthocyanins. In the wild bilberry (Vaccinium myrtillus L.), a clear increasing trend in anthocyanin biosynthesis has been reported toward northern latitudes of Europe, but studies related to altitude have given contradictory results. The present study focused on the anthocyanin composition in wild bilberries and highbush blueberry ...
    • Carotenoid metabolism during bilberry (Vaccinium myrtillus L.) fruit development under different light conditions is regulated by biosynthesis and degradation 

      Karppinen, Katja; Zoratti, Laura; Sarala, Marian; Carvalho, Elisabete; Hirsimäki, Jenni; Mentula, Helmi; Martens, Stefan; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-04-21)
      <b>Background:</b> Carotenoids are important pigments and precursors for central signaling molecules associated in fruit development and ripening. Carotenoid metabolism has been studied especially in the climacteric tomato fruit but the content of carotenoids and the regulation of their metabolism have been shown to be highly variable between fruit species. Non-climacteric berries of the genus ...
    • Compositional and morphological analyses of wax in northern wild berry species 

      Trivedi, Priyanka; Karppinen, Katja; Klavins, Linards; Kviesis, Jorens; Sundqvist, Petri; Nguyen, Nga; Heinonen, Esa; Klavins, Maris; Jaakola, Laura; Väänänen, Juha; Remes, Janne; Häggman, Hely (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-21)
      Aerial surfaces of plants are covered by a waxy cuticle protecting plants from excessive water loss and UV light. In the present study, composition and morphology of cuticular waxes of northern wild berry species bilberry (<i>Vaccinium myrtillus</i> L.), lingonberry (V. <i>vitis-idaea</i> L.), bog bilberry (V. <i>uliginosum</i> L.) and crowberry (<i>Empetrum nigrum</i> L.) were investigated. Scanning ...
    • Developmental and Environmental Regulation of Cuticular Wax Biosynthesis in Fleshy Fruits 

      Trivedi, Priyanka; Nguyen, Nga; Hykkerud, Anne Linn; Häggman, Hely; Martinussen, Inger; Jaakola, Laura; Karppinen, Katja (Journal article; Peer reviewed, 2019-04-19)
      The aerial parts of land plants are covered by a hydrophobic layer called cuticle that limits non-stomatal water loss and provides protection against external biotic and abiotic stresses. The cuticle is composed of polymer cutin and wax comprising a mixture of very-long-chain fatty acids and their derivatives, while also bioactive secondary metabolites such as triterpenoids are present. Fleshy ...
    • Genetic diversity and population structure of an important wild berry crop 

      Zoratti, Laura; Palmieri, Luisa; Jaakola, Laura; Häggman, Hely (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      The success of plant breeding in the coming years will be associated with access to new sources of variation, which will include landraces and wild relatives of crop species. In order to access the reservoir of favourable alleles within wild germplasm, knowledge about the genetic diversity and the population structure of wild species is needed. Bilberry (Vaccinium myrtillus) is one of the most ...
    • Light-controlled flavonoid biosynthesis in fruits 

      Zoratti, Laura; Karppinen, Katja; Escobar, Ana Luengo; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-10-09)
    • Modification of sunlight radiation through colored photo-selective nets affects anthocyanin profile in Vaccinium spp. berries 

      Zoratti, Laura; Jaakola, Laura; Häggman, Hely; Giongo, Lara (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-08-19)
      <p>Objectives In recent years, the interest on the effects of the specific wavelengths of the light spectrum on growth and metabolism of plants has been increasing markedly. The present study covers the effect of modified sunlight conditions on the accumulation of anthocyanin pigments in two Vaccinium species: the European wild bilberry (V. myrtillus L.) and the cultivated highbush blueberry ...
    • Monochromatic light increases anthocyanin content during fruit development in bilberry 

      Zoratti, Laura; Sarala, Marian; Carvalho, Elisabete; Karppinen, Katja; Martens, Stefan; Giongo, Lara; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
    • MYBA and MYBPA transcription factors co-regulate anthocyanin biosynthesis in blue-coloured berries 

      Karppinen, Katja; Lafferty, Declan J.; Albert, Nick W.; Mikkola, Nelli; McGhie, Tony K.; Allan, Andrew C.; Afzal, Bilal M.; Häggman, Hely; Espley, Richard V.; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-05)
      <p>We used blue berries of bilberry (Vaccinium myrtillus) to comprehensively characterise flavonoid-regulating R2R3 MYBs, which revealed a new type of co-regulation in anthocyanin biosynthesis between members of MYBA-, MYBPA1- and MYBPA2-subgroups. <p>VmMYBA1, VmMYBPA1.1 and VmMYBPA2.2 expression was elevated at berry ripening and by abscisic acid treatment. Additionally, VmMYBA1 and VmMYBPA1.1 ...
    • On the developmental and environmental regulation of secondary metabolism in Vaccinium spp. berries 

      Karppinen, Katja; Zoratti, Laura; Nguyenquynh, Nga; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-05-18)
      Secondary metabolites have important defense and signaling roles, and they contribute to the overall quality of developing and ripening fruits. Blueberries, bilberries, cranberries, and other Vaccinium berries are fleshy berry fruits recognized for the high levels of bioactive compounds, especially anthocyanin pigments. Besides anthocyanins and other products of the phenylpropanoid and flavonoid ...
    • Recognition of candidate transcription factors related to bilberry fruit ripening by de novo transcriptome and qRT-PCR analyses 

      Nguyen, Nga; Suokas, Marko; Karppinen, Katja; Vuosku, Jaana; Jaakola, Laura; Häggman, Hely (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-02)
      Bilberry (<i>Vaccinium myrtillus</i> L.) fruits are an excellent natural resource for human diet because of their special flavor, taste and nutritional value as well as medical properties. Bilberries are recognized for their high anthocyanin content and many of the genes involved in the anthocyanin biosynthesis have been characterized. So far, neither genomic nor RNA-seq data have been available for ...
    • Temperature has a major effect on the cuticular wax composition of bilberry (Vaccinium myrtillus L.) fruit 

      Trivedi, Priyanka; Klavins, Linards; Hykkerud, Anne Linn; Kviesis, Jorens; Elferts, Didzis; Martinussen, Inger; Klavins, Maris; Karppinen, Katja; Häggman, Hely; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-20)
      Cuticle is the first layer protecting plants against external biotic and abiotic factors and is responsive to climatic factors as well as determined by genetic adaptations. In this study, the chemical composition of bilberry fruit cuticular wax was investigated through a latitudinal gradient from Latvia (56◦N 24◦E) through Finland (65◦N 25◦E) to northern Norway (69◦N 18◦E) in two seasons 2018 ...